Compositions and methods for synthesizing nucleic acids

Details Compositions and methods for synthesizing nucleic acids Compositions and methods for synthesizing nucleic acids

A - Human Necessities

61

K

A61K 38/00 (2006.01) C07H 21/04 (2006.01) C07K 16/00 (2006.01) C07K 16/40 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2498130

Disclosed are compositions preferably for use in nucleic acid synthesis that include one or more anti-reverse transcriptase (RT) antibodies and/or one or more anti-DNA polymerase (DNAP) antibodies and/or single strand binding proteins (SSBs). Some of the disclosed compositions include one or more anti- DNAP antibodies and/or one or more anti-RT antibodies and one or more SSBs. Other disclosed compositions include two or more SSBs. The disclosed nucleic acid synthesis compositions also can include one or more DNAPs, one or more RTs, one or more nucleotides, one or more primers, and/or one or more templates. Also disclosed are methods for using such compositions in nucleic acid synthesis, amplification and sequencing, where various combinations of anti-RT antibodies, anti-DNAP antibodies and/or SSBs can improve the yield and/or homogeneity of primer extension products.

L'invention concerne des compositions destinées à être utilisées de préférence dans la synthèse d'acides nucléiques, qui comprennent un ou plusieurs anticorps anti-transcriptase inverse (RT) et/ou un ou plusieurs anticorps anti-ADN polymérase et/ou des protéines de liaison simple brin (SSB). Certaines compositions de l'invention comprennent un ou plusieurs anticorps anti-ADN polymérase et/ou un ou plusieurs anticorps anti-RT et une ou plusieurs protéines SSB. D'autres compositions de l'invention comprennent au moins deux protéines SSB. Lesdites compositions de synthèse d'acides nucléiques peuvent également comprendre une ou plusieurs ADN polymérases, une ou plusieurs RT, un ou plusieurs nucléotides, une ou plusieurs amorces, et/ou une ou plusieurs matrices. L'invention concerne également des procédés d'utilisation desdites compositions dans la synthèse, l'amplification et le séquençage d'acides nucléiques, selon lesquels diverses combinaisons d'anticorps anti-RT, anti-ADN polymérase et protéines SSB peuvent améliorer la production et/ou l'homogénéité des produits d'extension d'amorce.

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