Processes and vectors for amplification or expression of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/08 (2006.01)

Patent

CA 2439199

This invention discloses a biologically safe process of causing amplification and/or expression of one or more nucleic acid sequences of interest in a plant, plant tissue, plant cell or cell culture, characterized in that a plant cell is provided with at least two precursor vectors designed for undergoing processing by site-specific recombination in said cell, whereby due to said processing said plant cell is endowed with at least one replicon which provide(s) for said amplification and/or expression.

Cette invention concerne un procédé biologiquement sans danger consistant à provoquer l'amplification et/ou l'expression d'une ou plusieurs séquences d'acide nucléique d'intérêt dans un végétal, un tissu végétal, une cellule végétale ou une culture cellulaire, et se caractérisant par ce qu'une cellule végétale est utilisée avec au moins deux vecteurs précurseurs destinés à effectuer un traitement par recombinaison spécifique à un site dans ladite cellule. Du fait de ce traitement, ladite cellule végétale est pourvue d'au moins un réplicon assurant l'amplification et/ou l'expression.

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