Assay for glycosylated proteins

Details Assay for glycosylated proteins Assay for glycosylated proteins

G - Physics

01

N

G01N 33/53 (2006.01) G01N 33/66 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2482957

The invention provides a method for assaying for a protein having at least two isoforms having different glycosylation patterns, said method comprising: contacting a sample containing said protein with a carbohydrate-binding agent and a ligand capable of binding to at least two said isoforms, and detecting conjugates of said carbohydrate-binding agent and said protein and/or of said ligand and said protein.

L'invention concerne un procédé d'analyse d'une protéine présentant au moins deux isoformes qui possèdent des motifs de glycosylation différents. Ce procédé comprend les étapes consistant: à mettre en contact un échantillon comprenant ladite protéine avec un agent de liaison aux glucides et avec un ligand pouvant se lier auxdits isoformes; et à détecter des conjugués dudit agent de liaison aux glucides et de ladite protéine et/ou dudit ligand et de ladite protéine.

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