Stratified cartilage tissue and methods to engineer same

A - Human Necessities – 61 – K

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A61K 35/32 (2006.01) C12N 5/077 (2010.01) A61K 35/12 (2006.01)

Patent

CA 2460539

The present invention is directed to a cultured, stratified cartilage tissue that comprises a tissue-engineered, cohesive cartilage construct comprised of two or more cartilage layers, wherein each cartilage layer comprises chondrogenic cells having a chondrocytic phenotype corresponding to chondrocytes present in: a superficial-tangential zone of natural cartilage; a middle-transitional zone of natural cartilage; a deep-radial zone of natural cartilage; or a calcified cartilage zone of natural cartilage.

L'invention concerne un tissu cartilagineux stratifié cultivé qui comprend un produit de synthèse à base de tissu cartilagineux cohésif génétiquement manipulé comprenant deux couches cartilagineuses ou plus composée chacune de cellules chondrogéniques dont le phénotype chondrocytaire correspond aux chondrocytes présents dans: une zone superficielle tangentielle de cartilage naturel; une zone centrale transitionnelle de cartilage naturel; une zone radiale profonde de cartilage naturel; ou encore une zone cartilagineuse calcifiée de cartilage naturel.

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