Methods for isolating long fragment rna from fixed samples

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2691209

The present invention relates to methods for the extraction of long fragment RNA from fixed tissue specimens. In particular, the present invention relates to methods for the extraction of RNA from formalin-fixed paraffin-embedded tissue speci-mens for use in biologic applications, including assays based on oligonucleotide hybridization.

La présente invention concerne des procédés d'extraction d'ARN en longs fragments à partir de prélèvements tissulaires fixés. L'invention concerne en particulier des procédés d'extraction d'ARN à partir de prélèvements tissulaires inclus en paraffine et fixés au formol en vue d'une utilisation dans des applications biologiques, notamment dans des essais reposant sur l'hybridation d'oligonucléotides.

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