Noninvasive measurement of carotenoids in biological tissue

G - Physics – 01 – N

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G01N 21/25 (2006.01) G01N 21/65 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2726975

A method and apparatus are provided for the determination of carotenoid antioxidants and similar chemical compounds in biological tissue such as living skin. The method and apparatus provide a noninvasive, rapid, accurate, and safe determination of carotenoid levels which in turn can provide diagnostic information of the antioxidant status of tissue. Reflection spectroscopy is used to measure the concentrations of carotenoids and similar substances in tissue. White light is directed upon the area of tissue that is of interest. A small fraction of diffusively scattered light is collected and measured. The tissue is pressured to temporarily squeeze blood out of the measured tissue volume while the reflection spectrum is continuously monitored, displayed, and analyzed in near real time. After an optimal time period of typically 15 seconds, the influence of the dominating hemoglobin and oxyhemoglobin tissue absorptions on the reflection spectra are minimized.

L'invention porte sur un procédé et sur un appareil pour la détermination d'antioxydants caroténoïdes et de composés chimiques analogues dans un tissu biologique tel que la peau vivante. Le procédé et l'appareil permettent d'assurer une détermination non invasive, rapide, précise et sans danger des taux de caroténoïdes lesquels, à leur tour, peuvent fournir des informations de diagnostic sur l'état anti-oxydant du tissu. Une spectroscopie par réflexion est utilisée pour mesurer les concentrations de caroténoïdes et de substances analogues dans le tissu. Une lumière blanche est dirigée sur la zone de tissu qui est d'intérêt. Une petite fraction de lumière diffusée de façon diffuse est collectée et mesurée. Le tissu est pressuré pour exprimer temporairement le sang hors du volume de tissu mesuré tandis que le spectre de réflexion est surveillé en continu, affiché et analysé en temps presque réel. Après une période de temps optimale de typiquement 15 secondes, l'influence des absorptions dominantes dans le tissu d'hémoglobine et d'oxyhémoglobine sur les spectres de réflexion est rendue minimale.

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