Newly characterised oxalate oxidase and uses therefor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2107804

This invention pertains to methods for using oxalate oxidase in plant pathology. A substantially pure gene encoding the oxalate oxidase enzyme is elucidated. The expression product of the gene which can be stably incorporated into a foreign plant host has a unique profile including a pH optimum of 3.5, a positive heat stability, a single sub-unit of approximately 25 kilodal- ions and protease stability. The methodology of this invention exploiting oxalate oxidase for protection against oxalic acid en- compasses providing to a plant in need of oxalic acid protection an oxalic acid degrading enzyme in an amount sufficient to pro- tect the plant from the oxalic acid.

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