Methods of preparing a targeting vector and uses thereof

Details Methods of preparing a targeting vector and uses thereof Methods of preparing a targeting vector and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2463453

The present invention relates to providing methods for preparing a targeting construct for use in a targeting vector for gene targeting or homologous recombination. The invention also provides targeting vectors, and cells, plants and animals (including yeast) containing the vectors having predetermined modifications. The invention further provides plants and animals modified by the targeting vectors.The gene targeting methods used herein are based on transposon and recombination mediated procedures which provide for high throughput generation of deletions, which is amenable to semi automated production of knockout vectors.

L'invention concerne des méthodes de préparation d'une construction de ciblage à utiliser dans un vecteur de ciblage conçu pour cibler un gène ou une recombinaison homologue. L'invention porte également sur des vecteurs de ciblage, des cellules, des plantes et des animaux (dont la levure) contenant les vecteurs présentant des modifications prédéterminées. Elle se rapporte encore à des plantes et à des animaux modifiés par les vecteurs de ciblage. Les méthodes de ciblage de gène utilisées ici sont basées sur des procédures médiées par recombinaison ou par transposons qui permettent la génération en grande quantité de délétions, pouvant faire l'objet d'une production semi-automatisée de vecteurs de <= knocking out >=.

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