Dna hybridization probes for identification of mycobacteria

Details Dna hybridization probes for identification of mycobacteria Dna hybridization probes for identification of mycobacteria

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2016553

DNA HYBRIDIZATION PROBES FOR IDENTIFICATION OF MYCOBACTERIA ABSTRACT OF THE INVENTION A nucleic acid sequence specific for genomic nucleic acid of Mycobacterium tuberculosis is disclosed. This sequence is from M. tuberculosis and can hyhridize with certain members of the tuberculosis complex of myco- bacterial e.g. M. tuberculosis, M. bovis and the Montreal strain of M. bovis (Calmette Bacille-Guerin). This invention also pertains to DNA fragments identified by amplification of mycobacterial nucleic acid by the polymerase chain reaction (PCR) technique using oligode- oxyribonucleotide primers derived from this isolated nucleic acid sequence of M. tuberculosis. DNA fragments amplified in this manner will specifically hybridize with nucleic acid from M. tuberculosis. The invention also pertains to diagnostic methods such as hybridization assays which employ the nucleic acid sequences and to kits for performing the assays.

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