Method for preparing comprehensive anti-surface antibody...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/02 (2006.01) A23K 1/18 (2006.01) A23L 1/305 (2006.01) A61K 39/395 (2006.01) A61P 31/00 (2006.01) C07K 16/12 (2006.01)

Patent

CA 2747696

A method for preparing comprehensive antibodies against whole proteins that are expressed on the surface of a microorganism and the comprehensive antibodies obtained by the method are provided. The method for preparing comprehensive antibodies against whole proteins that are expressed on the surface of a microorganism comprises: (a) treating a microorganism with a protein crosslinking and immobilization reagent and thus immobilizing proteins expressed on the surface of the microorganism via intramolecular crosslinking; (b) administering the microorganism as an immunogen immobilized using the protein crosslinking and immobilization reagent to an animal; and (c) obtaining antibodies from the animal.

L'invention concerne un procédé de production d'un anticorps global contre la totalité des protéines apparaissant à la surface d'un micro-organisme, ainsi qu'un anticorps global obtenu à l'aide dudit procédé. Le procédé de production d'un anticorps global comprenant un anticorps contre la totalité des protéines apparaissant à la surface d'un micro-organisme consiste, (a) grâce au traitement d'un micro-organisme à l'aide d'un réactif de fixation par réticulation de protéines, à fixer par réticulation intramoléculaire des protéines apparaissant à la surface du micro-organisme susmentionné; (b) à injecter à un animal, en tant qu'immunogène, le micro-organisme susmentionné, fixé à l'aide dudit réactif de fixation par réticulation de protéines; (c) à obtenir un anticorps à partir de l'animal.

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