Enhanced transfection system

Details Enhanced transfection system Enhanced transfection system

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/045 (2006.01) C07K 14/16 (2006.01) C07K 14/54 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/26 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2420771

A mammalian cell gene expression vector system comprising (a) an episomal maintenance system (b), a strong promoter/enhancer, (c) a protein transactivation system and (d) DNA coding for a heterologous protein. The episomal maintenance and protein transactivation systems can include sub- elements located on the same or different plasmids within the cell expression system.

L'invention concerne un système de vecteur d'expression génique de cellule mammalienne comprenant (a) un système de maintenance épisomique (b), un promoteur/activateur puissant, (c) un système de transactivation de protéine et (d) de l'ADN codant une protéine hétérologue. Les systèmes (a) et (c) peuvent comprendre des éléments secondaires situés sur des plasmides identiques ou différents dans le système d'expression cellulaire.

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