System to separate frozen-thawed spermatozoa into...

Details System to separate frozen-thawed spermatozoa into... System to separate frozen-thawed spermatozoa into...

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) A01K 67/00 (2006.01) A01K 67/033 (2006.01) A01N 1/02 (2006.01) C12M 1/00 (2006.01) C12N 5/02 (2006.01) C12N 15/00 (2006.01)

Patent

CA 2468772

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm sampels collected forma male mammel.Specifically, techniques to uniformily stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y- chromosome bearing populations having high purity.

L'invention concerne des dispositifs, des compositions et des méthodes de manipulation, de dissociation, de conditionnement et d'utilisation de spermatozoïdes (1) pouvant provenir d'échantillons de sperme congelé collecté chez un mammifère mâle. Cette invention concerne de manière spécifique des techniques, permettant de colorer (2) de façon uniforme l'ADN des spermatozoïdes même s'ils proviennent d'un sperme préalablement congelé, et des techniques de dissociation permettant de dissocier et d'isoler les spermatozoïdes de qualité supérieure porteurs d'un chromosome X de ceux porteurs d'un chromosome Y.

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