Remodeling and glycopegylation of fibroblast growth factor...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/18 (2006.01) A61K 38/18 (2006.01) C07K 14/50 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2585758

The present invention relates to mutants of Fibroblast Growth Factor (FGF), particularly FGF-20 and FGF-2 1, which contain newly introduced N-linked or O- linked glycosylation site(s). The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

L'invention concerne des mutants du facteurs de croissance des fibroblastes (FGF), en particulier FGF-20 et FGF-21, qui renferment un(des) site(s) de glycosylation à liaison N ou à liaison O. L'invention concerne en outre les séquences de codage polynucléotidiques pour les mutants, des cassettes d'expression comprenant les séquences de codage, des cellules exprimant les mutants, et des procédés de production des mutants. Enfin elle concerne des compositions pharmaceutiques comprenant les mutants ainsi qu'un procédé d'utilisation de ces mutants.

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