Selective detection of bordetella species

Details Selective detection of bordetella species Selective detection of bordetella species

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2759681

A process for detecting Bordetella spp. nucleic acid in a biological sample includes producing an amplification product(s) by amplifying one or more Bordetella spp. in a multiplex single chamber PCR assay, and measuring said amplification product(s) to detect or distinguish Bordetella spp. in the biological sample. Also provided are reagents and methods for detecting and distinguishing Bordetella spp. from each other and other bacteria or viruses. A kit is provided for detecting and quantifying one or more Bordetella spp. in a biological sample.

Un procédé de détection de l'acide nucléique de Bordetella sp. dans un échantillon biologique comprend la production d'un ou de produits d'amplification par amplification d'un ou de plusieurs Bordetella sp. dans un dosage PCR multiplex en chambre simple, et de mesure desdits produits d'amplification pour détecter ou distinguer Bordetella sp. dans l'échantillon biologique. L'invention concerne également des réactifs et des procédés de détection et de distinction des Bordetella sp. les uns des autres et d'autres bactéries ou virus. L'invention concerne un kit pour la détection et la quantification d'un ou de plusieurs Bordetella sp. dans un échantillon biologique.

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