Epitope tagging system

Details Epitope tagging system Epitope tagging system

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 1/107 (2006.01) C07K 1/22 (2006.01) C07K 7/06 (2006.01) C07K 9/00 (2006.01) C07K 14/14 (2006.01) C07K 16/10 (2006.01) C07K 16/44 (2006.01) C07K 19/00 (2006.01) C12N 15/46 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01) G01N 33/532 (2006.01)

Patent

CA 2229540

Hexapeptides which function as epitope tags when linked to molecules of interest are described. Epitope tags such as those having the sequence Q-Y, F, H or W-P-A, S or V - L or V - T, L, V or Q are useful for purifying a protein of interest, or for studying and characterising a molecule of interest. Preferably QYPALT, QYPSLL, QYPSLQ, QFPALL, QYPVLV and QYPSLT. Most preferably QYPALT.

L'invention porte sur des hexapeptides ayant la fonction d'épitopes marqueurs lorsqu'ils se fixent à des molécules considérées. Des marqueurs de ce type, tels que ceux présentant la séquence Q-Y,F,H ou W-P-A,S ou V-L ou V-T,L, V ou Q, ou mieux QYPALT, QYPSLL, QYPSLQ, QFPALL, QYPVLV et QYPSLT ou idéalement QYPALT, servent à la purification d'une protéine considérée, ou, à l'étude ou à la caractérisation d'une molécule considérée.

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