Multiplexed fluorescent detection in microfluidic devices

Details Multiplexed fluorescent detection in microfluidic devices Multiplexed fluorescent detection in microfluidic devices

G - Physics

01

N

G01N 21/64 (2006.01)

Patent

CA 2373537

An optical detection and orientation device is provided comprising housing having an excitation light source. An optical element for reflecting the excitation light to an aspherical lens and transmitting light emitted by a fluorophore excited by said excitation light, a focusing lens for focusing the emitted light onto the entry of an optical fiber, which serves as a confocal aperture, and means for accurately moving said housing over a small area in relation to a channel in a microfluidic device. The optical detection and orientation device finds use in identifying the center of the channel and detecting fluorophores in the channel during operations involving fluorescent signals.

L'invention concerne un dispositif de détection et d'orientation optique qui comprend un boîtier pourvu d'une source de lumière d'excitation, un élément optique pour réfléchir la lumière d'excitation en direction d'une lentille asphérique et transmettre la lumière émise par un fluorophore excité par ladite lumière d'excitation, une lentille de focalisation servant à focaliser la lumière émise sur l'entrée d'une fibre optique, qui sert d'ouverture confocale, ainsi qu'un système servant au déplacement de précision, à travers une petite zone, par rapport à un canal dans un dispositif microfluidique. Le dispositif optique de détection et d'orientation peut servir pour identifier le centre du canal et détecter les fluorophores dans le canal lors des opérations avec des signaux fluorescents.

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