Plant vde genes and methods related thereto

C - Chemistry – Metallurgy – 12 – N

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C12N 15/52 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2236267

DNA sequences encoding plant vde enzymes are provided herein. The sequences may be joined to heterologous DNA sequences for use as probes and in DNA constructs to modify the genotype of a host organism. DNA constructs and methods are provided to modify a host cell phenotype by altering the amount of photoprotection enzyme present in the host cell. In plastid containing host cells, zeaxanthin levels and sensitivity to light can be modified through alterations in the level of vde enzymes.

Sequences ADN codant pour des enzymes de violaxanthine déepoxydase des végétaux. Ces séquences peuvent être réunies à des séquences ADN hétérologues pour être utilisées comme sondes dans des constructions d'ADN afin de modifier le gènotype de l'organisme hôte. L'invention porte également sur des constructions d'ADN et des procédés permettant de modifier le phénotype des cellules hôtes par modification de la quantité d'enzymes de photoprotection présents dans les cellules hôtes. Dans des cellules hôtes contenant des plastides, les taux de zéaxanthine et la sensibilité à la lumière peuvent être modifiés par des modifications du taux de violaxanthine déepoxydases.

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