Chemical, biochemical and biological processing in thin films

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/00 (2006.01) B01J 19/00 (2006.01) B01J 19/08 (2006.01) C07K 1/04 (2006.01) C12M 1/42 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2223496

An optical trap is used to translate a particle through a thin film coating on an optically-flat surface. Preferably, the thin film coating is heterogeneous and the optical trap is used to move the particle through a succession of different regions of the thin film coating where different chemical, biochemical and/or biological processes take place. Examples of chemical, biochemical and/or biological processes that might be implemented in accordance with the invention include the following: oligonucleotide synthesis and sequencing, peptide synthesis and sequencing, carbohydrate synthesis and sequencing, combinatorial library synthesis and screening, conventional (i.e., Sanger or Maxam-Gilbert) DNA sequencing, or single-molecule DNA sequencing. In one embodiment of the invention, reaction products are left behind as the particle is moved through the thin film coating. Advantageously, these products can be identified by suitable means.

On utilise un piège optique pour translater une particule à travers le revêtement mince d'une surface optique plane. Le revêtement est de préférence hétérogène et le piège optique sert à déplacer la particule dans une succession de régions différentes du revêtement où se déroulent différents processus chimiques, biochimiques et biologiques tels que la synthèse et le séquençage d'oligonucléotides, la synthèse et le séquençage de peptides, la synthèse et le séquençage d'hydrates de carbone, la synthèse et le criblage de bibliothèques combinatoires, le séquençage usuel d'ADN (par exemple de Sanger ou de Maxam-Gilbert), ou le séquençage de molécules isolées d'ADN. Dans l'une des variantes de l'invention, des produits de réaction sont laissés sur place alors que la particule se déplace à travers le revêtement en couche mince. Ces produits présentent l'avantage de pouvoir être identifiés par des moyens appropriés.

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