Methods of analysis and labeling of protein-protein...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/48 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2443842

We have discovered a new method to analyze and characterize complex cell signaling networks. The method is based on specific binding of protein-protein interaction modules to a single type of protein or a mixture of proteins. The method utilizes a number of different protein-protein interaction domains as probes or sensors for the signaling state of the system under investigation.

L'invention concerne une nouvelle méthode permettant d'analyser et de caractériser des réseaux complexes de signalisation cellulaire. Cette méthode repose sur la liaison spécifique de modules d'interaction protéine-protéine à un type unique de protéine ou à un mélange de protéines. Ladite méthode utilise un certain nombre de différents domaines d'interaction protéine-protéine en tant que sondes ou détecteurs de l'état de signalisation du système, en cours d'investigation.

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