Transformed plants with improved growth characteristics due...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/12 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2536650

The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.

L'invention concerne un procédé destiné à modifier les caractéristiques de croissance des plantes, par modulation de l'expression dans une plante, d'un acide nucléique CDK de type B et/ou modulation de l'activité et/ou de niveaux dans une plante, d'une protéine CDK de type B. L'invention concerne également des plantes transgéniques ayant des caractéristiques de croissance modifiées, lesdites plantes ayant une expression modulée d'acide nucléique CDK de type B et/ou une activité modulée et/ou des niveaux modulés dans une plante d'une protéine CDK de type B par rapport à une expression, une activité et/ou des niveaux dans des plantes de type sauvage correspondantes. L'invention concerne également un nouveau procédé de criblage pour l'identification de CDK mutants ayant une activité CDK améliorées par rapport à des CDK correspondants non mutés. L'invention concerne en outre un nouveau procédé de criblage pour l'identification de CDK non actifs, capables de se lier à des CKI (inhibiteurs de kinase dépendant de la cycline). Enfin, l'invention concerne des CDK mutants, obtenus conformément aux procédés de criblage selon l'invention.

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