Method of gene transfer using retrotransposons

Details Method of gene transfer using retrotransposons Method of gene transfer using retrotransposons

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2072581

2072581 9207950 PCTABS00013 A process for the transfer and/or expression of any gene into cells, organisms or species is disclosed. The process involves isolating the gene, introducing the gene into a vector comprised of at least one retrotransposon genetic element to provide a hybrid gene. The hybrid gene is introduced into a donor cell capable of packaging and transmitting the retrotransposon to other cells, organisms or species. The hybrid gene is transferred to a recipient cell wherein the hybrid gene replicates by reverse transcription and is inserted into the recipient cell's genome. The gene is preferably expressed as RNA and/or protein, giving the phenotype of the gene.

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