Substantially complete ribozyme libraries

Details Substantially complete ribozyme libraries Substantially complete ribozyme libraries

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2335390

The present invention provides a high complexity substantially complete hairpin ribozyme library having a randomized recognition sequence, packaged in a vector and operably linked to a promoter suitable for high level expression in a wide variety of cells. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.

L'invention concerne une banque de ribozymes en épingle à cheveux sensiblement complète et très complexe, possédant une séquence de reconnaissance aléatoire, empaquetée dans un vecteur et liée activement à un promoteur convenant à une expression de haut niveau dans une grande variété de cellules. Ladite banque est utilisée dans une grande variété de protocoles de sélection pour l'identification, l'isolement et la caractérisation d'ARN cibles connus ou non, de sorte que les effets phénotypiques dudit clivage soient révélés et que les produits géniques produisant lesdits effets soient identifiés.

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