Microbial reduction of benzazepine and benzothiazepine...

C - Chemistry – Metallurgy – 12 – P

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C12P 17/14 (2006.01) C07D 223/16 (2006.01) C07D 281/10 (2006.01) C07D 403/06 (2006.01) C12P 17/10 (2006.01) C12P 17/16 (2006.01)

Patent

CA 2032091

HA466a MICROBIAL REDUCTION OF BENZAZEPINE AND BENZOTHIAZEPINE DERIVATIVES Abstract of the Disclosure A novel process comprises reducing a benzazepine or benzothiazepine at the 3-position in d-cis configuration by treatment with reductase- supplying microorganisms or enzymes derived therefrom. The process can be catalyzed in a single stage by growing microbial cultures or in a two-stage fermentation/transformation by resting cell-suspensions. The enzymes derived from the microorganisms can be used in free state or immobilized form. The microorganisms and enzymes catalyzes the specific reduction with 90 to 99% conversion efficiency to 99% or greater optical purity of the desired enantiomer.

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