Three-color flow cytometry with automatic gating function

Details Three-color flow cytometry with automatic gating function Three-color flow cytometry with automatic gating function

G - Physics

01

N

G01N 15/14 (2006.01) G01N 21/47 (2006.01) G01N 21/64 (2006.01) G01N 33/487 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2141664

A flow cytometry based system (100) is provided which uses a three color reagent panel (an isotypic control, a helper/T-cytotoxic suppressor/total T-cell reagent, and a NK/B/T-cell reagent) in conjunction with a cytometer which permits direct absolute cell counts. Additionally, a system for automatic accurate selection of subpopulations is provided.

Un système (100) basé sur la cytométrie en flux utilise un tableau de réactifs de trois couleurs (une commande isotype, un réactif de la totalité des lymphocytes T/suppresseur cytotoxique-T/auxiliaire cytotoxique, et un réactif des lymphocytes NK/B/T) conjointement avec un cytomètre qui permet des comptages directs, absolus des cellules. De plus, l'invention concerne un système de sélection précise, automatique des sous-population de cellules.

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