Electrofusion chamber

Details Electrofusion chamber Electrofusion chamber

C - Chemistry, Metallurgy

12

N

C12N 15/02 (2006.01) C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 13/00 (2006.01)

Patent

CA 2329945

An apparatus is provided for producing cell electrofusion (20) wherein the cells to be fused are directed to and retained on one side of a substrate (34) and between the electrodes (30, 32). Thus, a significant portion of the cells are in close cell-to-cell contact so that dielectrophoresis is not needed before electrofusion. In a preferred embodiment, the substrate is porous (34) and the cells are held onto the porous substrate by means of a vacuum (38).

L'invention concerne un appareil pour réaliser une électrofusion de cellules (20), dans lequel les cellules à traiter sont dirigées et retenues sur un côté d'un substrat (34) et entre les électrodes (30, 32). Ainsi, une partie importante des cellules sont étroitement liées les unes aux autres, ce qui rend inutile une diélectrophorèse avant l'électrofusion. Dans une forme de réalisation préférée, le substrat est poreux (34) et les cellules sont retenues sur ledit substrat poreux par un procédé réalisé sous vide (38).

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