C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2422736
A method of forming a complex consisting of a double-stranded nucleic acid and oligonucleotide(s) characterized by comprising: the step of mixing a double- stranded nucleic acid with at least one oligonucleotide to give a reaction mixture, wherein the oligonucleotide is a chimeric oligonucleotide containing at least a member selected from among deoxyribonucleotides and nucleotide analogs and a ribonucleotide and having a sequence substantially complementary to the base sequence of one of the strands of the double-stranded nucleic acid as described above; and the step of incubating the reaction mixture to form a complex under such conditions that the above-described double-stranded nucleic acid is not denatured.
Procédé de formation d'un complexe constitué d'un acide nucléique à double brin et d'un ou plusieurs oligonucléotides, qui consiste à mélanger un acide nucléique à double brin avec au moins un oligonucléotide pour obtenir un mélange de réaction. Ledit oligonucléotide est un oligonucléotide chimérique contenant au moins un élément sélectionné parmi des désoxyribonucléotides et des analogues de nucléotides, et un ribonucléotide. Cet oligonucléotide possède une séquence essentiellement complémentaire à la séquence des bases de l'un des brins de l'acide nucléique à double brin décrit ci-dessus. Ledit procédé consiste ensuite à faire incuber le mélange de réaction pour former un complexe dans des conditions telles que l'acide nucléique à double brin n'est pas dénaturé.
Enoki Tatsuji
Kato Ikunoshin
Kobayashi Eiji
Sagawa Hiroaki
Tomono Jun
Fetherstonhaugh & Co.
Takara Bio Inc.
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