A method for identifying cytotoxic t-cell epitopes

G - Physics – 01 – N

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G01N 33/554 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2251781

A method for isolating bio-active molecules from complex, rationally designed oligopeptide libraries which elicit cytolytic activity from cloned cytotoxic T lymphocytes (CTLs). The method allows the simultaneous screening of multiple CTL lines against indexed peptide libraries synthesized on solid phase support. Preferably decoding is not dependent on the presence of residual peptide and does not employ peptide sequencing. The method to identify CTL- reactive oligopeptides yields products of therapeutic value such as vaccines in treating cancers, viral diseases, and autoimmune diseases, as well as to identify useful clinical diagnostic reagents with a reduction in assay time and increase in throughput.

Méthode permettant d'isoler des molécules bioactives à partir de bibliothèques d'oligopeptides complexes et conçues rationnellement qui provoquent l'activité cytolytique de lymphocytes T cytotoxiques clonés. La méthode permet le criblage simultané de multiples lignées de ces lymphocytes par rapport à des bibliothèques de peptides indexées, synthétisées sur un support en phase solide. De préférence, le décodage n'est pas dépendant de la présence de peptide résiduel et ne nécessite pas de séquençage peptidique. Ladite méthode d'identification d'oligopeptides réagissant aux lymphocytes T cytotoxiques donne des produits ayant une valeur thérapeutique, tels que des vaccins pour le traitement des cancers, des affections virales et des maladies auto-immunes. Elle permet d'identifier des réactifs diagnostiques utiles sur le plan clinique avec réduction du temps d'analyse et augmentation de la production.

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