Rh blood group antigen compositions and methods of use

Details Rh blood group antigen compositions and methods of use Rh blood group antigen compositions and methods of use

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) A61K 35/14 (2006.01) A61K 38/00 (2006.01) C07K 1/00 (2006.01) C07K 5/00 (2006.01) C07K 7/00 (2006.01) C07K 14/00 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 16/34 (2006.01) C07K 17/00 (2006.01) C12N 5/00 (2006.01) G01N 33/53 (2006.01) G01N 33/537 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01) G01N 33/545 (2006.01) G01N 33/549 (2006.01) G01N 33/551 (2006.01) G01N 33/552 (2006.01) G01N 33/553 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2269092

Disclosed are novel protein and peptide compositions comprising soluble and bound forms of immunologically-active blood group antigens including mammalian Rh antigens. In preferred embodiments methods for the isolation and purification of serologically-active human Rh antigens such as D, c, C, E, and e are disclosed. Also disclosed are methods for the adsorption of immunologically-active Rh antigens to solid supports. Diagnostic kits, methods, and devices for the detection of Rh antibodies in clinical and non- clinical samples are also disclosed. Devices, compositions and methods for the isolation, purification and quantitation of anti-Rh antibodies from solution are also provided.

L'invention concerne de nouvelles compositions de protéines et de peptides, comprenant des formes solubles et liées d'antigènes de groupes sanguins immunocompétents, dont des antigènes Rh de mammifère. Dans des modes de réalisation préférés, des procédés destinés à l'isolation et à la purification d'antigènes Rh sérologiquement actifs, tels les antigènes D, c, C, E et e, sont décrits. Des procédés destinés à l'adsorption d'antigènes Rh immunocompétents sur des supports solides sont également décrits. Des kits de diagnostic, des procédés et des dispositifs de détection d'anticorps Rh dans des échantillons cliniques et non cliniques sont également décrits. Des dispositifs, des compositions et des procédés destinés à l'isolement, à la purification et au dosage d'anticorps anti-Rh à partir d'une solution sont également prévus.

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