C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 9/16 (2006.01) C12N 15/63 (2006.01)
Patent
CA 2743711
This invention relates to cloning and sequencing of thermotolerant phytase gene from Non-K12 Escherichia coli strain, ATCC 9637, phytase gene expression in Escherichia coli expression system, codon usage optimized and expression in Pichia pastoris, Pichia methanolica and Kluyeromyces lactis. The high level yield and thermotolerant enzyme was produced from fermentation of Pichia pastoris with optimized codon of phytase gene.
La présente invention concerne le clonage et le séquençage du gène de la phytase thermotolérante de la souche d'Escherichia coli non-K12 ATCC 9637, l'expression du gène de la phytase dans un système d'expression pour Escherichia coli, l'utilisation optimisée des codons et l'expression dans Pichia pastoris, Pichia methanolica et Kluyeromyces lactis. L'enzyme thermotolérante et à haut niveau de rendement a été produite par fermentation de Pichia pastoris avec optimisation des codons du gène de la phytase.
Chen Caifang
Jin Weigang
Li Renkuan
Ye Xiuyun
Fujian Fuda Biotech Co. Ltd.
Ridout & Maybee Llp
LandOfFree
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