G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/535 (2006.01) C12Q 1/42 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2001206
A composition contains levamisole and a substrate for alkaline phosphatase in a high pH buffer including 2-amino-2-methyl-1-propanol. The composition may be included in a kit of materials useful in performing an immunoassay in which intestinal alkaline phosphatase is the label. The invention includes a method for detection of a viral antigen by an immunoassay including intestinal alkaline phosphatase as the label and the composition of the invention to provide stabilized levamisole to inhibit nonintestinal alkaline phosphatase.
Une composition contient du lévamisole et un substrat pour la phosphatase alcaline dans un tampon à pH élevé de 2-amino-2-méthyl-1-propanol. La composition peut être employée dans une trousse de réactifs utilisée pour réaliser un immunodosage dans lequel la phosphatase alcaline intestinale est marquée. L'invention comprend une méthode de détection d'un antigène viral par un immunodosage comprenant de la phosphatase alcaline intestinale comme marqueur et la composition de l'invention pour fournir du lévamisole stabilisé pour inhiber la phosphatase alcaline non intestinale.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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