Three-dimensional tissue equivalent using macromass culture

Details Three-dimensional tissue equivalent using macromass culture Three-dimensional tissue equivalent using macromass culture

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) A61P 17/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2678695

The present invention provides a three-dimensional tissue equivalent for in-vivo and in-vitro uses. The three dimensional tissue equivalent of the present invention is a non-contractile cellular sheet cultured over a porous scaffold using a macromass culturing technique, for example where the cellular sheet is entirely on one side of a porous sponge. In one embodiment, the present invention provides a dermal wound dressing that comprises a high density cellular sheet of dermal fibroblast cells.

La présente invention propose un équivalent de tissu tridimensionnel pour des utilisations in vivo et in vitro. L'équivalent de tissu tridimensionnel de la présente invention est une feuille cellulaire non contractile mise en culture sur un échafaudage poreux à l'aide d'une technique de culture de macromasse, par exemple, où la feuille cellulaire est entièrement sur un côté d'une éponge poreuse. Dans un mode de réalisation, la présente invention propose un pansement pour plaies dermiques qui comporte une feuille cellulaire haute densité de cellules fibroblastes dermiques.

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