Enzyme capture assay

Details Enzyme capture assay Enzyme capture assay

G - Physics

01

N

G01N 33/573 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2118709

2118709 9305398 PCTABS00020 A method of determining human secretory acetylcholinesterase (sAChE) in a test sample, which method comprises contacting a ligand which is capable of binding sAChE and which is not an antibody with the sample and determining wether human sAChE has bound to the ligand; and a method of determining an enzyme in a test sample, which method comprises: i) bringing into contact the sample and a ligand which is immobilised on the surface of a solid or matrix support, which ligand is: a) a substrate or substrate analogue of the enzyme; b) an effector or effector analogue of the enzyme; c) a co-factor or co-factor analogue of the enzyme; d) a co-enzyme or a co-enzyme analogue of the enzyme; or e) a prosthetic group or prosthetic group analogue of the enzyme; and ii) determining whether the enzyme has bound to the ligand.

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