A group of novel enantioselective microbial nitrile...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/14 (2006.01) C07K 14/195 (2006.01) C12N 1/20 (2006.01) C12N 9/78 (2006.01) C12P 13/02 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2648557

The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E. C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.

La présente invention concerne un polynucléotide ou une paire de polynucléotides codant pour une enzyme ayant une activité de nitrile hydratase (NHase) [E. C. 4.2.1.84]. Elle concerne en outre un vecteur et un hôte comprenant le polynucléotide ou la paire de polynucléotides selon l'invention, ainsi que des procédés pour les produire. En outre, l'invention concerne une paire de polypeptides ou une protéine de fusion ayant une activité de NHase, un anticorps qui se lie spécifiquement à la paire de polypeptides ou à la protéine de fusion, une amorce ou une sonde qui s'hybride spécifiquement en conditions stringentes au polynucléotide selon l'invention ou à l'un ou l'autre des polynucléotides de la paire, une composition comprenant le polynucléotide ou la paire de polynucléotides, la paire de polypeptides ou la protéine de fusion, l'anticorps et/ou une ou plusieurs amorces ou sondes de l'invention, ainsi qu'un procédé de production d'amides, comprenant la conversion énantiosélective de nitriles.

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