A method of flow cytometric determination of telomerase...

Details A method of flow cytometric determination of telomerase... A method of flow cytometric determination of telomerase...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G01N 15/10 (2006.01)

Patent

CA 2206007

There are provided a quantitative and simple method of determining telomerase activity and a method of detecting a telomerase positive cell, said methods comprising: (i) a step of conducting elongation reaction of telomerase substrate with telomerase in cells; (ii) a step of subjecting the resultant elongation reaction product to base binding chain reaction in cells; and (iii) a step of quantitatively determining fluorescent light derived from a reaction product in cells using flow cytometry.

Méthode quantitative simple servant à déterminer la télomérase et à détecter les cellules présentant ce phénomène. En voici les étapes : i) création d'une réaction d'élongation de substrat de télomérase avec la télomérase des cellules, ii) exposition du produit de la réaction d'élongation à la réaction en chaîne de liaison de base dans les cellules et iii) détermination quantitative (par cytométrie de flux) de la lumière fluorescente émise par le produit de la réaction dans les cellules.

Affiliated with

Also associated with

Rating

A method of flow cytometric determination of telomerase... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with A method of flow cytometric determination of telomerase..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and A method of flow cytometric determination of telomerase... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1871535