Improved compositions and methods for cdna synthesis

Details Improved compositions and methods for cdna synthesis Improved compositions and methods for cdna synthesis

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2497420

Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided.Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

Cette invention concerne des méthodes de fabrication de molécules d'ADNc en vue de l'amplification d'ARN par réaction en chaîne de la polymérase (PCR) et de la préparation de banques d'ADNc. L'invention porte également sur des kits de fabrication de molécules d'ADNc. Sont en outre décrites des compositions comprenant des mélanges de réactifs, dont des transcriptases inverses, tampons, co-facteurs et autres composants pouvant être utilisés immédiatement pour la transformation d'ARN en ADN c et RT PCR (à transcriptase inverse) sans dilution ou adjonction d'autres composants. Ces compositions conviennent bien, seules ou sous forme de kits, pour la synthèse de l'ADNc ou l'amplification d'acides nucléiques (telle que la réaction en chaîne de la polymérase) ou pour toute méthode faisant intervenir des transcriptases inverses dans divers domaines : recherche, applications médicales, diagnostiques, médico-légales ou agricoles.

Affiliated with

Also associated with

Rating

Improved compositions and methods for cdna synthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Improved compositions and methods for cdna synthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Improved compositions and methods for cdna synthesis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1872080