Vector for introducing molecules in eukaryotic cells and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/79 (2006.01) A61K 48/00 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2435094

The invention concerns a vector for introducing a molecule (X) having at least an amino-terminal end into a eukaryotic cell, characterised in that it consists of an association of a cell incorporating module enabling membrane translocation, and an uniquitin module or other related peptide capable of conjugation, said vector having a carboxy-terminal end designed to be fused by peptide bond to the amino-terminal end of said molecule (X). The invention is characterised in that said vector is rapidly destroyed after separation from said molecule (X). The invention also concerns any molecule vectorized by such a vector.

L'invention concerne un vecteur pour l'introduction d'une molécule (X) présentant au moins une extrémité amino-terminale dans une cellule eucaryote caractérisé en ce qu'il est constitué par l'association d'un module d'incorporation cellulaire permettant la translocation membranaire, et d'un module ubiquitine ou autre peptide conjugable apparenté, ledit vecteur présentant une extrémité carboxy-terminale destinée à être fusionnée par liaison peptidique à l'extrémité amino-terminale de ladite molécule (X) caractérisé en ce que ledit vecteur est rapidement détruit après séparation de ladite molécule (X). L'invention couvre également toute molécule vectorisée par un tel vecteur.

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