Methods for production of receptor and ligand isoforms

Details Methods for production of receptor and ligand isoforms Methods for production of receptor and ligand isoforms

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A61K 38/17 (2006.01) A61K 38/18 (2006.01) A61K 48/00 (2006.01) C07K 14/475 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/54 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) C12N 9/12 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2628959

Provided are methods for production of cell surface receptor (CSR) and ligand isoforms. In particular, isoform fusions that a precursor sequence for secretion, processing and intracellular trafficking are provided. Nucleic acid molecules encoding the fusions are expressed in a host cell and the encoded and partially or completely processed encoded CSR or ligand isoforms is produced in the cell culture medium. The resulting polypeptide optionally includes an epitope tag for the detection and/or purification thereof.

La présente invention se rapporte à des procédés permettant de produire des isoformes de récepteurs de surface cellulaire (CSR) et de ligands. L'invention a trait en particulier à des fusions d'isoformes, qui contiennent une séquence précurseur permettant la sécrétion, le traitement et le trafic intracellulaire. Des molécules d'acide nucléique codant lesdites fusions sont exprimées dans une cellule hôte, et les isoformes de CSR ou de ligands codées et partiellement ou totalement traitées sont produites dans le milieu de culture cellulaire. Le polypeptide obtenu contient éventuellement un marqueur épitope destiné à sa détection et/ou purification.

Affiliated with

Also associated with

Rating

Methods for production of receptor and ligand isoforms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for production of receptor and ligand isoforms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for production of receptor and ligand isoforms will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1873462