Use of suppressor trna's to regulate cytotoxicity during the...

Details Use of suppressor trna's to regulate cytotoxicity during the... Use of suppressor trna's to regulate cytotoxicity during the...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C07K 14/015 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/63 (2006.01) C12N 15/861 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2340335

A method for regulating the expression of a toxic protein in a cell destined to produce a recombinant gene product is disclosed. The method relies on the introduction of a termination codon into the coding sequence of the toxic protein and complementation with a suppressor tRNA.

Cette invention a trait à une technique de régulation de l'expression d'une protéine toxique dans une cellule destinée à produire un produit génique de recombinaison. La technique repose sur l'introduction d'un codon de terminaison dans la séquence codante de la protéine toxique et sur une complémentation à l'aide d'un ARN de transfert suppresseur.

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