Coleopteran-toxic polypeptide compositions and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2371442

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed .delta.- endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

L'invention concerne de nouveaux polypeptides insecticides et des compositions contenant ces polypeptides, des fragments peptidiques de ceux-ci et des anticorps spécifiques associés. L'invention concerne également des vecteurs, des cellules hôtes transformées et des plantes transgéniques qui contiennent des segments d'acides nucléiques codant pour les polypeptides d'endotoxine .delta. décrits. L'invention concerne également des procédés d'identification de polypeptides et de polynucleotides apparentés, des procédés de fabrication et d'utilisation de cellules transgéniques contenant ces séquences polynucléotidiques, ainsi que des procédés de lutte contre une population d'insectes, tel que le doryphore de la pomme de terre, les larves de la chrysomèle des racines du maïs du sud et du maïs de l'ouest, et des procédés pour conférer à une plante une résistance à une espèce d'insecte voulue.

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