Process for the separation and purification of sialyl...

Details Process for the separation and purification of sialyl... Process for the separation and purification of sialyl...

C - Chemistry, Metallurgy

12

N

C12N 9/10 (2006.01)

Patent

CA 2051374

Specific sialyl transferases (ST) are important enzymes in the manufacture of carbohydrate moieties useful for pharmaco- logical purposes and for diagnostic and clinical procedure. The invention offers simplified methods to isolate highly specific forms of these ST enzymes by taking advantage of the affinity of the ST for its acceptor substrate in the presence of the sialyl- CMP analogs, such as CDP. Specifically exemplified are isolation of the alpha 2,3-ST, specific for Lec or LacNAc, and alpha 2,6-ST, specific for LacNAc.

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