Methods for producing thrombin

Details Methods for producing thrombin Methods for producing thrombin

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) A61K 38/48 (2006.01) C07K 14/39 (2006.01) C12N 1/19 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 9/74 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2125783

Methods are disclosed for producing thrombin. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. The DNA constructs gen- erally include the following operably linked elements: a transcriptional promoter, DNA sequence encoding a gla-domainless pro- thrombin, and a transcriptional terminator. Thrombin precursors produced from transformed or transfected host cells are activat- ed either in vivo or in vitro.

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