C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C12N 15/10 (2006.01)
Patent
CA 2483338
The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
La présente invention concerne une méthode de création de bibliothèques d'ADN comprenant une bibliothèque de promoteurs artificiels et/ou une bibliothèque de sites de liaison ribosomique modifiés, et de transformation des cellules hôtes bactériennes avec la bibliothèque pour obtenir une population de clones bactériens avec une gamme de niveaux d'expression pour un gène chromosomique d'intérêt.
Cervin Marguerite A.
Soucaille Philippe
Valle Fernando
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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