Production of vanillin

Details Production of vanillin Production of vanillin

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) A01H 5/00 (2006.01) A23L 1/03 (2006.01) A23L 1/22 (2006.01) A23L 2/56 (2006.01) C12N 1/19 (2006.01) C12N 1/20 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/88 (2006.01) C12N 15/53 (2006.01) C12N 15/82 (2006.01) C12P 7/24 (2006.01) C12P 7/42 (2006.01)

Patent

CA 2250043

A method of producing vanillin comprising the steps of: (1) providing trans- ferulic acid or a salt thereof; and (2) providing trans-ferulate: CoASH ligase activity (enzyme activity I), trans-feruloyl SCoA hydratase activity (enzyme activity II), and 4-hydroxy-3-methoxyphenyl-.beta.-hydroxy-propionyl SCoA (HMPHP SCoA) cleavage activity (enzyme activity III). Conveniently the enzymes are provided by Pseudomonas fluorescens Fe3 or a mutant or derivative thereof. Polypeptides with enzymes activities II and III and polynucleotides encoding said polypeptides. Use of said polypeptides or said polynucleotides in a method for producing vanillin.

Procédé de production de vanilline comprenant les étapes suivantes: (1) on prend un acide trans-férulique ou un sel dudit acide; (2) on expose ledit acide à du transférulate: activité de CoASH ligase (activité enzymatique I), activité de trans-féruloyl SCoA hydratase (activité enzymatique II), et activité de clivage de 4-hydroxy-3-méthoxyphényl-.beta.-hydroxy-propionyl SCoA (HMPHPSCoA) (activité enzymatique III). Les enzymes sont aisément obtenues à partir de Pseudomonas fluorescens Fe3 ou une forme mutante ou dérivée de ces derniers. L'invention concerne également des polypeptides aux activités enzymatiques II et III et des polynucléotides codant ces polypeptides ainsi que leur utilisation dans un procédé de production de vanilline.

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