C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/11 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2473683
Aptamer based fluorescent reporters that function based on a switch from DNA/DNA duplex conformation to DNA/target conformation are provided. The DNA/DNA duplex is formed between the aptamer DNA sequence and an oligonucleotide carrying a reporter moiety. When the aptamer target is present, the aptamer assumes a tertiary structure for binding to the target. The formation of the tertiary structure forces the dissociation of the duplex structure and a signal is generated. The signal is preferably a fluorescent signal due to spatial separation of a fluorophore/quencher pair.
L'invention concerne des rapporteurs fluorescents à base aptamère fonctionnant sur la base d'un passage d'une conformation duplex ADN/ADN à une conformation ADN/cible. Le duplex ADN/ADN est formé entre la séquence d'ADN aptamère et un oligonucléotide portant un groupe fonctionnel rapporteur. Lorsque la cible aptamère est présente, ledit aptamère prend une structure tertiaire afin de lier la cible. La formation de la structure tertiaire provoque la dissociation de la structure duplex et un signal est produit. Ledit signal est de préférence un signal fluorescent provoqué par la séparation spatiale d'une paire fluorophore/extincteur.
Li Yingfu
Nitiu Razvan
Gowling Lafleur Henderson Llp
Mcmaster University
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