Method and kit for effecting screening and immunogenic...

A - Human Necessities – 61 – K

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

A61K 39/395 (2006.01) A61K 31/164 (2006.01) A61K 31/337 (2006.01) A61K 31/555 (2006.01) A61K 31/704 (2006.01) A61K 38/02 (2006.01) A61K 38/17 (2006.01) A61K 38/19 (2006.01) A61K 38/44 (2006.01) A61K 45/00 (2006.01) A61P 35/00 (2006.01) A61P 37/04 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2665771

Anthracyclines-treated tumor cells are particularly effective in eliciting an anti-cancer immune response, where the rDNA-damaging agents, such as etoposide and mitomycin C do not induce immunogenic cell death. Anthracyclines induce the rapid, pre-apoptotic translocation of calreticulin (CRT) and/or ERP57 to the cell surface. Knock down of CRT and/or ERP57 suppressed the phagocytosis of anthracyclines-treated tumor cells by dendritic cells and abolished their immunogenicity in mammals, such as mice. The anthracyclines-induced CRT and/or ERP57 translocation was mimicked by inhibition of the protein phosphatase1/GADD34 complex. Administration of recombinant calreticulin, and not recombinant ERP57, or inhibitors of protein phosphatase1/GADD34 restored the immunogenicity of cell death elicited by etoposide and mitomycin C, and enhanced their antitumor effects in vivo. These data identify calreticulin and/or ERP57 as a key feature determining anti-cancer immune responses and delineate a possible strategy for immunogenic chemotherapy. This invention demonstrates that calreticulin exposure dictates also sperm-egg fusion. This invention includes also the using of recombinant CRT and/or ERP57 or the inducers of the translocation of CRT and/or ERP57 protein to treat sterility. The detection of the translocation of CRT and/or ERP57 is a good way to screen new immunogenic molecules and compounds.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method and kit for effecting screening and immunogenic... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method and kit for effecting screening and immunogenic..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method and kit for effecting screening and immunogenic... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1886136

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.