Genes encoding carotenoid compounds

Details Genes encoding carotenoid compounds Genes encoding carotenoid compounds

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/90 (2006.01) C12N 15/31 (2006.01) C12N 15/53 (2006.01) C12N 15/61 (2006.01) C12N 15/62 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2528574

A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and &bgr;-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

Selon l'invention, un groupe de gènes biosynthétiques caroténogéniques unique a été isolé de la souche DC404 de Panteoa agglomerans, lequel groupe présente une organisation génétique crtE-idi-crtY-crtI-crtB-crtZ. Les gène contenus dans ce groupe codent la géranylgéranyl pyrophosphate (GGPP) synthétase (CrtE), l'isopentényl pyrophosphate isomérase (Idi), la lycopène cyclase (CrtY), la phytoène désaturase (CrtI), la phytoène synthase (CrtB), et la .beta.-carotène hydroxylase (CrtZ). Le groupe de gènes, les gènes et leurs produits sont utilisés dans la conversion du farnesyl pyrophosphate en caroténoïdes. L'invention porte également sur des vecteurs contenant ces segments d'ADN, sur des cellules hôtes contenant les vecteurs et sur des procédés permettant de produire ces enzymes par une technique de recombinaison d'ADN dans des organismes hôtes transformés.

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