Phosphoramidite derivatives, their preparation and the use...

Details Phosphoramidite derivatives, their preparation and the use... Phosphoramidite derivatives, their preparation and the use...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07F 9/24 (2006.01) C07F 9/547 (2006.01) C07F 9/6561 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2081320

2081320 9117169 PCTABS00008 Phosphoramidite derivatives of formulae (V) or (XII), wherein X is a reporter group and Y and Z are protecting groups. The reporter group X may comprise any hapten or other detectable moiety and examples include boitin, dinitrophenyl, dansyl and fluoresceinyl. There may be a linker arm, of variable length, between the reporter group and the rest of the molecule. Examples of the protecting groups, Y and Z, include 4,4'-dimethoxytrityl, trifluoroacetyl and fluorenylmethoxycarbonyl (Fmoc). The phosphoramidite derivatives are useful in the single or multiple labelling of synthetic oligonucleotides. Processes for the preparation of these phosphoramidite derivatives are also disclosed.

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