C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) C07K 16/18 (2006.01) C07K 16/40 (2006.01) C12N 9/76 (2006.01) C12N 15/06 (2006.01) C12Q 1/34 (2006.01) G01N 33/573 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2339048
The dynamics in vivo of trypsin, in particular, canine trypsin and/or canine trypsin-like immunoreactants have attracted attention in analyzing the relation thereof with various diseases such as pancreatic diseases. Since trypsin has two subclasses, i.e., the cationic type and the anionic type and, therefore, occurs in various forms, it is also required to assay individual types thereof. To satisfy the above requirement, a method for accurately quantitating trypsin or a method for detecting trypsin is provided. A monoclonal antibody immunologically reacting specifically with trypsin can be obtained by the cell fusion method by using as an immunogen trypsin, in particular, canine trypsin or a region containing its specific amino acid sequence or neighborhood thereof. By using the thus obtained monoclonal antibody as an assay reagent, trypsin and/or trypsin-like immunoreactants occurring in various forms can be quickly and accurately assayed by, in particular, the sandwich assay method, etc. Moreover, the clinical meaning of the ratio of trypsin, etc. in diseases (acute pancreatitis, chronic pancreatitis, pancreatic cancer, renal insufficiency, extrapancreatic hyposecretion, etc.) can be clarified thereby.
La dynamique in vivo de la trypsine, singulièrement de la trypsine canine et/ou des immunoréactifs de type trypsine, à attirer l'attention sur l'analyse du rapport desdites substances avec diverses maladies dont les affections du pancréas. Comme la trypsine possède deux sous-classes, le type cationique et le type anionique, il faut également analyser ces deux types. C'est pourquoi la présente invention concerne un procédé de quantification précise et de détection de la trypsine. Un anticorps monoclonal réagissant au plan immunologique spécifiquement avec la trypsine peut être obtenu par hybridation somatique au moyen d'une trypsine immunogène, en particulier une trypsine canine ou une région renfermant une séquence d'acides aminés ou son voisinage. L'utilisation de l'anticorps ainsi obtenu comme réactif permet, en particulier grâce à la méthode de d'essai en sandwich, de doser rapidement et précisément la trypsine et/ou diverses formes 'immunoréactifs de type trypsine. De plus, cette méthode permet de clarifier la signification du rapport de la trypsine, etc. dans diverses pathologies, telles que la pancréatite aiguë, la pancréatite chronique, le cancer du pancréas, l'insuffisance rénale ou l'hyposécrétion extrapancréatique.
Ashida Yoshinori
Waritani Takaki
Yamada Takatsugu
Daiichi Fine Chemical Co. Ltd.
Fetherstonhaugh & Co.
Fuji Yakuhin Kogyo Kabushiki Kaisha
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