Microfluidic apparatus for performing gel protein...

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01) B81B 1/00 (2006.01)

Patent

CA 2434836

The invention relates to an apparatus for performing gel protein extractions and methods of using the apparatus. In one embodiment of the apparatus, a fluidic channel (1) is fed through a housing cover (3) and is held in position by a sleeve (2). The fluidic channel is held in place over a specific position on a gel (5). The gel may have undergone 1D or 2D gel electrophoresis. Once gel electrophoresis has been completed, the gel is washed, stained, and equilibrated, and then is placed in the apparatus housing (8) with electrical contact to a ground electrode (7). The fluidic channels are then placed over and put in contact with the desired spot on the gel, either by manual or robotic controls. An electric potential is then aplied at the outlet reservoir (9) from a high voltage power supply (10) through an electrode (4).

L'invention se rapporte à un appareil permettant de réaliser des extractions de protéine de gel et les procédés d'utilisation de cet appareil.

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