Composition and methods using nitroxides to avoid oxygen...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/805 (2006.01) A61J 1/14 (2006.01) A61K 9/127 (2006.01) A61K 35/18 (2006.01) A61K 38/38 (2006.01) A61K 38/42 (2006.01) A61K 39/395 (2006.01) A61K 45/06 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) A61K 49/20 (2006.01) A61M 1/34 (2006.01) C07K 14/76 (2006.01) C07K 16/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2169178

This invention relates to the technical field of compositions and processes to alleviate oxygen toxicity based on the addition of nitroxides to physiologically compatible macromolecules. In particular, hemoglobin-based red cell substitutes are described featuring stable nitroxide free radicals for use in cell-free hemoglobin solutions, encapsulated hemoglobin solutions, stabilized hemoglobin solutions, polymerized hemoglobin solutions, conjugated hemoglobin solutions, nitroxide-labelled albumin, and nitroxide-labelled immunoglobulin. The formulations described herein interact with free radicals, act as antioxidant enzyme-mimics, and alleviate oxidative stress and oxygen- related toxicity.

L'invention concerne le domaine technique des compositions et procédés destinés à atténuer la toxicité de l'oxygène par adjonction de nitroxydes à des macromolécules physiologiquement compatibles. On décrit notamment des substituts de globules rouges à base d'hémoglobine qui présentent des radicaux libres nitroxyde stables destinés à être utilisés dans des solutions d'hémoglobine dépourvues de globules, d'hémoglobine encapsulée, d'hémoglobine stabilisée, d'hémoglobine polymérisée, d'hémoglobine conjuguée ou avec de l'albumine ou des immunoglobulines marquées par des nitroxydes. Les formulations décrites permettent une interaction avec des radicaux libres, interviennent comme simulateurs d'enzymes antioxydantes et atténuent les contraintes dues à l'oxydation et la toxicité liée à l'oxygène.

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